由于把双端的read须要判断方向,因此第一步的时候就已经用根据建库的方式,duTP建库第一条链作反向互补,在read后面加上/1,第二条链加上/2,SMART建库的时候,第二条链作反向互补加上/1,第二条链加上/2。由于作这个转化的时候在read名字后面多了一个space号,因此出现了上面相似的问题spa
@E00511:189:HC3TYCCXY:5:1101:3051:1959/1
GGGCATGGGATTTACTTATTCAGCAAAGGGTTGCCTTTACCACCCAGAAGGATTTTGGAAGAGAGGCCAGAGAGATAAGATGGGCTCATGCTCAGAGGACTTTGCATGGATTACAACCACCAGACACAAAACTGTTTGGTGACAGAA
+
<F7FJFFJJJAJJJFJJJJJJJJJFAJJJJJJJJJJJJJJJJJFFFFJJJJJA<A<JJJJJJJJJJJJJJJJJJJJJJJJJFJJJJJAJJJFJJJJJJJJFAAJFJFJJJ-FJFJJJ-A-FJJJJJJJJJJJJJJJJFJJFJJJJJJ
@E00511:189:HC3TYCCXY:5:1101:3133:1959/2
GGTGTAGCAGGAACTGGACCGTCCTCCGCGAGGTCTGATAATGCCTCGATTGGTGTATTGCCTGCTAATTTGTCTTCTAATAGAGAGAGCAAGCCCATTTCAGAGAACCCGGAAGAAGAGAACGATGGAGTCCGGGATATTCATGTA
+
FFJAJJJJJJJJJJJJJJJJJJJJJJJJJJJJJFFJJJJJJJJJ7AJJFAFFJJF-FJJJJJJJJ<JJJJJJAJJJJFJJJJJJJJJJJ<JJJFJJJFJJJJJJJJJJFJJJJJJJFAFJJJJJJJJJJJFJJJJJJJJJFJJJJJJ